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Antibody Validation and Establishment of SOCS1 and SOCS3 Upregulation Kinetics. Splenocytes, isolated from C57BL/6 mice, were harvested and treated in vitro with LPS (5000 μg/ml) or IFN‐γ (500 U) at the time points 6, 12, 24 and 48 h in the presence or absence of anti‐CD3 (4 μg/ml).

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